The pair looked to be having a rather heated discussion as Alesha Dixon stood awkwardly to one side.
Ant McPartlin and Amanda Holden have been spotted in what looks like a furious row at yesterday's Britain's Got Talent auditions.
Amanda looked as though she was berating the host and neither of them looked happy during the photocall.
The pair were seen having a very heated discussion at The Lowry theatre in Salford, where auditions are being held to pick the next crop of talented performers for this year's series.
Dressed in a white floor-length gown with a peplum white jacket on top, Amanda's face was contorted as she appeared to direct her ire at newly single Ant.
The Geordie presenter, 42, leaned in close to Amanda as she vented, then stood back with a blank-looking face as an unidentified woman interjected and laid her hand on Amanda's arm, perhaps to calm her down.
Amanda, 47, seemed to take no notice of fellow judge Alesha Dixon, who stood awkwardly to the side during the discussion.
Ant's TV partner Declan Donnelly was standing to Ant's left during the conversation and appeared to make no move to stop them.
But a source close to Ant denied there was anything wrong between the two.
"Amanda and Ant are just chatting. This picture was taken while they were posing on front of hundreds of fans and press. There simply wasn't any form of heated discussion whatsoever."
Ant's rep slammed the report, calling it "absolute rubbish".
"Ant and Amanda were not having any form of argument. They were literally having a conversation," he added.
After their exchange, Amanda stood well away from Ant for the BGT photocall.
She was sandwiched in between Alesha and show boss Simon Cowell , while Ant was placed right at the far end.
Ant, who last month announced he was divorcing wife of 11 years Lisa Armstrong, looked downcast as he walked in past the screaming fans and entered the theatre.
He kept his hands in his pockets as he strode in, only switching on a smile when he was in front of the cameras.